Researchers identify pathway that regulates lipid synthesis and contributes to tumor survival
Cancer cells undergo numerous changes that promote cell survival and continued growth. One of the hallmark changes of cancer cells is the increased breakdown of the sugar glucose, which when combined with poor blood flow, leads to the development of a highly acidic surrounding tumor environment. To survive in this environment, cancer cells undergo adaptations, such as activation of the self-degradation and recycling process called autophagy, and the accumulation of lipid fat droplets. Lipid droplets play an important role in the regulation of energy, metabolism and signal transduction; however, scientists are unsure how lipid droplets accumulate in tumor cells or what their impact is on cancer survival and progression.
Moffitt researchers performed a series of laboratory experiments with cell lines and mouse models to improve their understanding of lipid droplets in cancer. They discovered that breast cancer cell lines grown in acidic conditions accumulate intracellular lipid droplets that express the lipid droplets biomarker protein PLIN2. Lipid droplets formed when a protein in the cellular membrane called OGR1 sensed the presence of the acidic environment. OGR1 subsequently activated downstream signal transduction through the proteins phospholipase C and PI3K/AKT, which led to the formation of lipid droplets from the products of amino acids that had undergone a metabolic breakdown into smaller components.
Next, the team wanted to assess the biological effects of lipid droplets and OGR1 on cancer. Interestingly, the acid sensing receptor OGR1 is highly expressed in breast tumors and strongly associated with disease progression. They demonstrated that by targeting OGR1, they could reduce levels of lipid droplets, inhibit stress responses and cell growth in acidic conditions, and decrease tumor growth in mice. Additionally, the researchers discovered that high expression of the lipid droplets biomarker PLIN2 was associated with shorter survival and disease progression in breast cancer patients.
These combined observations suggest that lipid droplets formation mediated by OGR1 is an important contributor to tumor development and may be a potential target for anti-cancer drugs.
“Recent studies have established reprogramming of lipid metabolism as an emerging hallmark of many cancers, providing opportunities for therapeutic targeting. Many lipid inhibitors are being investigated as anticancer drugs in clinical trials. Our studies provide a strong foundation for future investigations that will lead to a more detailed characterization of the role of OGR1 in ER stress response, autophagy and lipogenesis in animal models and human breast tumors,” explained Smitha Pillai, Ph.D., lead study author and a research scientist in the Cancer Physiology Department at Moffitt.
This study was supported by the National Institutes of Health (R010-17536-99-02, R01-DK105346, P41-GM122698, 5U2C-DK119889, P30-CA076292), the Florida Department of Health Bankhead-Coley Cancer Research Program, the Florida Breast Cancer Foundation, the McKnight Brain Institute at the National High Magnetic Field Laboratory’s Advanced Magnetic Resonance Imaging and Spectroscopy Facility (National Science Foundation Cooperative Agreement DMR-1644779) and the European Research Council Consolidator Grant “Survive” (723997).